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 biologicalcontrols.com - Microbial Challenge Study

 
 MICROCON® MAP 800M

 


1205 INDUSTRIAL HIGHWAY ~ PO BOX 514 ~ SOUTHAMPTON, PA ~ 215 355-3900

Microbial Challenge Study of the MICROCON® MAP 800 M Air Purifier

The following study was performed by the Q. C. pharmaceutical microbiology
department from the dates Monday, September 8th until Monday, September 15th, 1997 for Biological Controls, Eatontown, NJ.

Procedure

The MICROCON® MAP 800 M air purifier was challenged with a known concentration of the following five microorganisms;

1. Aspergillis niger ATCC # 16404
2. Candida albicans ATCC # 10231
3. Pseudomonas aeruginosa ATCC # 9027
4. Staphylococcus aureus ATCC # 6538
5. Serratia marcesens ATCC # 14756

The unit was allowed to run with the UV lights on for a minimum of two hours prior to challenge in order for it to clean itself. Ten membrane filters (47 mm, 0.45um) were then placed on the unit, five on the iar intake screen and five post HEPA filters just below the series of UV lights. A known concentration of bacteria or fungi was then sprayed onto the intake screen with the unit in full operation with and without the UV light engaged. The unit was allowed to continue running for a period od fifteen minutes after the spraying at which time the filters were especially removed and placed onto petri dishes containing either Tryptiucase Soy Agar (bacteria) or Sabouraud Dextrose Agar (fungi). Bacteria plates were incubated at 35 +/- 2° C. for 48 hours while fungi plates were incubated at 25° C. for up to five days. In addition, after removal of the membrane filters, the unit was allowed to run with the UV light on for a period of eighteen hours at which time a series of six swabs were taken in different locations (three from the intake screen area and three from the post HEPA filter area.) One organism was tested per day. The results are recorded on the following pages. Please note that TNTC is an abbreviation for “too numerous to count.”

 


1205 INDUSTRIAL HIGHWAY ~ PO BOX 514 ~ SOUTHAMPTON, PA ~ 215 355-3900


Date Organism Conc. of organisms
sprayed on Intake
Recovered colonies on mebrane filters
MICROCON® MAP 800 M Air Purifier
Intake Post HEPA
9-8-97 A niger 2 x 10* TNTC
TNTC
TNTC
TNTC
TNTC
<1
<1
<1
<1
<1
9-9-97 C albicans 2 x 10* TNTC
TNTC
TNTC
TNTC
TNTC
<1
<1
<1
<1
<1
9-10-97 P aeruginosa 4 x 10* TNTC
TNTC
TNTC
TNTC
TNTC
<1
<1
<1
<1
<1
9-11-97 S aureus 3 x 10* TNTC
TNTC
TNTC
TNTC
TNTC
<1
<1
<1
<1
<1

 


1205 INDUSTRIAL HIGHWAY ~ PO BOX 514 ~ SOUTHAMPTON, PA ~ 215 355-3900

 

Date Organism Conc. of organisms
sprayed on Intake
Recovered colonies on mebrane filters
MICROCON® MAP 800 M Air Purifier
Intake Post HEPA
9-12-97 S marcescens 6 x 10* TNTC
TNTC
TNTC
TNTC
TNTC
<1
<1
<1
<1
<1


SWABS
Recovered colonies per swab

Organism   Intake   Post HEPA filter
Top Middle Bottom Top Middle
Bottom
A niger
C albicans
P aeruginosa
S aureus
S marcescens
15
0
0
1
2
11
1
0
0
8
3
0
1
2
1
<1
<1
<1
<1
<1
<1
<1
<1
<1
<1
<1
<1
<1
<1
<1

Vincent G Iuzzolina



Director of Microbiology



 

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